Liposomes Part B - download pdf or read online

Liposomes Part B - download pdf or read online

By Colowick S.P., Kaplan N.O.

Liposomes are mobile buildings made of lipid molecules. vital as a mobile version within the examine of uncomplicated biology, liposomes also are utilized in scientific functions akin to drug supply and virus reviews.

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Extra info for Liposomes Part B

Example text

H. Nielson, A. M. Judd, S. K. Han, W. Cho, P. J. Sims, and J. D. Bell, J. Biol. Chem. 274, 11494 (1999). 60 R. Kikuchi-Yanoshita, R. Yanoshita, I. Kudo, H. Arai, T. Takamura, K. Nomoto, and K. Inoue, J. Biochem. (Tokyo) 114, 33 (1993). [2] liposomes in the study of PLA2 activity 33 and the removal/replacement of calcium, the required cofactor for these enzymes. 12,63 It should be noted that cation-free sPLA2 does have the capacity to bind a phospholipid interface, depending of the nature of the phospholipids present.

The presence and mobility of water molecules in different depths of the membrane depends on the order and mobility of lipid molecules in the membrane. 3 mol% of the total lipid concentration in the chloroform solution before drying. Liposomes should be kept dark during both the preparation steps and storage. Alternatively, they can be incorporated after liposome formation by mixing a small volume of a concentrated stock of the probe in dimethyl sulfoxide with a suspension of liposomes at the mole ratio described above.

Salesse, Biochim. Biophys. Acta 1023, 365 (1990). 84 Assays of hydrolysis by thin-layer chromatography or the fatty acid-binding proteins and the use of laurdan fluorescence and the two-photon microscopy technique have provided promising results. The simplicity of erythrocytes compared with nucleated cells and the considerable background knowledge available make erythrocytes a good choice for efforts to apply lessons from liposomes to biological membranes. The future of combined approaches such as those described here with liposome membranes of various compositions and simple biological membranes should provide answers to important questions regarding the selective action of sPLA2 toward some, but not all, cell membranes.

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