By Thomas J. McIntosh (auth.), Thomas J. McIntosh (eds.)
In the prior numerous years major consciousness has been given to the research of the houses and services of lateral microdomains (rafts) in organic membranes. As defined within the evaluation bankruptcy of this booklet, in addition to within the introductions to the various different chapters, there are various basic un- swered questions about the composition, constitution, dynamics, or even the very life of those membrane rafts. hence, numerous subtle concepts were used to check intact cellphone membranes, in addition to version s- tems composed of particular lipids and proteins regarded as in rafts. The analyses of organic membranes have supplied special information on intact microdomains, and the complementary stories of version structures have given severe insights at the roles of particular lipid-lipid and lipid-protein interactions in raft formation and serve as. The chapters during this ebook supply exact info on sleek equipment which are at present being hired to review lipid rafts.
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Additional resources for Lipid Rafts
Tortorella, L. , and Brown, D. A. (1995) Characterization of proteins in detergent-resistant membrane complexes from Madin-Darby canine kidney epithelial cells. Biochemistry 34, 16,161–16,170. 22. Mattingly, R. R. (2002) Mitogen-Activated Protein Kinase Signaling in DrugResistant Neuroblastoma Cells, in Cancer Cell Signaling: Methods and Protocols, (Terrian, D. , Totowa, NJ, pp. 71–84. 23. , and Sobota, A. (2003) Insights into the association of FcγRII and TCR with detergent-resistant membrane domains: Isolation of the domains in detergent-free density gradients facilitates membrane fragment reconstitution.
2 should be generated. The points are then fit to a sigmoidal function. , Encinitas, CA) has been used in the lab. The Tm is the point at which the absolute value of the slope of F/Fo vs temperature is at a maximum. Figure 2 shows an example of an experiment in which the difference in ordered to liquid-disordered Tm for two different lipid mixtures is readily apparent. 5°C for the dioleoyl PCcontaining mixture and 42°C for the diphytanoyl PC-containing mixture. 38 Bakht and London 4. Notes 1. Tempo can be dissolved in water.
London, E. (2002) Insights into lipid raft structure and formation from experiments in model membranes. Curr. Opin. Struct. Biol. 12, 480–486. 7. Fastenberg, M. , Brown, D. , and London, E. (2003) Exclusion of a transmembrane-type peptide from ordered-lipid domains (rafts) detected by fluorescence quenching: extension of quenching analysis to account for the effects of domain size and domain boundaries. Biochemistry 42, 12,376–12,390. 8. Abrams, F. S. and London, E. (1992) Calibration of the parallax fluorescence quenching method for determination of membrane penetration depth: refinement and comparison of quenching by spin-labeled and brominated lipids.