By M.A. Nasser Hajibagheri
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Extra resources for Electro Microscopy Methods and Protocols (Methods in Molecular Biology Vol 117)
For simplicity, epoxy resin (Epon) embedding is described in this chapter; other resins are detailed in Glauert (2) and Chapters 6 and 7. Ultramicrotomy and staining ultrathin sections are dealt with briefly; for a detailed account of the procedure and trouble-shooting (3). The ultrathin sec- From: Methods in Molecular Biology, vol. 117: Electron Microscopy Methods and Protocols Edited by: N. , Totowa, NJ Page 2 tions are collected on grids for examination. , copper, nickel, and gold and are available in different designs including square mesh, hexagonal mesh, and parallel bars.
3. Remove cells by scraping from the support using Parafilm-coated spatula or other appropriately shaped implement. 3. and Notes 1 and 5). 6 Preparation of Epoxy Resins (Epon) There are several epoxy resins to choose from that have different viscosities. , Spurr resin have a carcinogenic component and are useful for hard material like bone but should be used and disposed of with care. For routine work, Epon is recommended by the author whose lab has solved many of the problems encountered by new users.
25 per page, is paid directly to the Copyright Clearance Center at 222 Rosewood Drive, Danvers, MA 01923. For those organizations that have been granted a photocopy license from the CCC, a separate system of payment has been arranged and is acceptable to Humana Press Inc. 25]. Printed in the United States of America. Nasser Hajibagheri. 117) Includes index. ) ; 117. 2. 28'25--dc21 DNLM/DL 98-37685 for Library of Congress CIP Page v Preface Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field.