Differentiated Cells in Aging Research - download pdf or read online

Differentiated Cells in Aging Research - download pdf or read online

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KASTEN Many of the discussions of this important work have not mentioned that Wieland and Scheuing (1921) also prepared the same kind of derivatives from Doebner’s violet that they prepared from pararosaniline. The two dyes are similar in that they are triphenylmethanes. Of greater importance here is the fact that each contains at least one amino group. This is discussed more fully in the section concerning Schiff-type reagents. Wieland and Scheuing showed that the N-sulfinic acid of Doebner’s violet may be produced with SO, and that this compound is also a reagent for aldehydes.

Other phenomena result if Schiff’s solution is heated for a short time to remove only part of the SOz. The solution becomes colored and stays colored while hot but, when cooled, it gradually becomes colorless again. This process may be repeated several times with the same solution (Kastle, 1909). , 1927). Both of these suggested reactions are shown on page 22; it should be kept in mind that the second and third steps of the hydrolytic reaction scheme are unlikely to occur. Wertheim (1922) claimed that his Schiff’s reagent was more sensitive after having been boiled for 1 minute, and he noted the color changes described above.

The curve is shifted to a higher wavelength, just as with the conventional Schiff’s reagent. Data from Kasten (19%). Unaware initially of the reports cited above, Kasten (1956a, 1958a, 1959) made an extensive survey of dyes to find new Schiff-type reagents, to e m o n s t r a t e by standard cytochemical method? the aldehyde specificity of such reagents, to utilize these colorimetric reagents in multiple-staining techniques, and to use such reagents to study cytochemical polyaldehyde reaction mechanisms.

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