By David W. Murhammer (auth.), David W. Murhammer (eds.)
Baculovirus Expression Protocols is an in depth consultant for utilizing the baculovirus expression vector process (BEVS) and/or insect cells to supply recombinant proteins. techniques for the advance and creation of baculovirus pesticides at either laboratory and massive scale are defined within the form of step by step structure that the equipment in Molecular Biology™ sequence helped standardize. This publication is a one-stop resource for info on baculoviruses, and comprises sections on utilizing converted baculoviruses to precise genes in mammalian cells, utilizing Drosophila mobile traces, separating new mobile strains, and constructing serum-free medium. particular functions, together with utilizing baculovirus and bug mobile approach to review apoptosis, also are mentioned. This up-to-date and accelerated version of Baculovirus Expression Protocols offers an ideal lab guide for biochemists, molecular biologists, biochemical engineers, and others for utilizing the BEVS or insect phone strains to supply recombinant proteins.
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Additional resources for Baculovirus and Insect Cell Expression Protocols
16, 3635–3654. 139 Mans, R. M. W. and Knebel-Mörsdorf, D. (1998) In vitro transcription of pe38/ 139. Polyhedrin hybrid promoters reveals sequences essential for recognition by the baculovirus-induced RNA polymerase and for the strength of very late viral promoters. J. Virol. 72, 2991–2998. 140 Weyer, U. and Possee, R. D. (1989) Analysis of the Autographa californica 140. nuclear polyhedrosis virus p10 gene. J. Gen. Virol. 70, 203–208. 141 Roelvink, P. , van Meer, M. M. , de Kort, C. A. , Possee, R.
These vectors have been of particular use in assembling structures in insect cells that are com- 42 Kelly, King, and Possee posed of more than one protein (170). Insect cells are also competent in accomplishing many of the posttranslational processes required when producing proteins, which have their origins in eukaryotic cells, so the products are normally biologically active. The original method for producing recombinant baculoviruses required the native polyhedrin gene to be replaced with the heterologous coding sequences, thus deriving a polyhedrin-negative virus.
Klinge-Roode, E. , van Lent, J. W. , and Vlak, J. M. (1989) 37. Construction and analysis of an Autographa californica nuclear polyhedrosis virus mutant lacking the polyhedral envelope. Virology 173, 98–108. 38 Russell, R. L. , Pearson, M. , and Rohrmann, G. F. (1991) Immunoelectron 38. microscopic examination of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus-infected Lymantria dispar cells: time course and localization of major polyhedrin-associated proteins. J. Gen. Virol. 72, 275–283.